Coding
aiiA

Part:BBa_C0060:Design

Designed by: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita D. Marinescu and Alexander D. Wissner-Gross   Group: Antiquity   (2003-01-31)


autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 99
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_C0060 insert contains open reading frame (nucleotides 49-801) of the GeneBank sequence AF196486 followed by the LVA tag and two double stop codons inserted in the BioBrick prefix and suffix flanking regions. The original stop codon was TAG and in the present sequence it was substituted by TAATAA.

Source

Bacillus sp. 240B1 putative metallohydrolase (aiiA) gene.

  • [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Search&db=nucleotide&term=AF196486 Genbank AF196486]
  • [http://www.pir.uniprot.org/cgi-bin/upEntry?id=Q9L8R8_9BACI UniProt Q9L8R8]


Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia

carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000).

References

  • Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia
  • carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). <a href="#">http://www.pnas.org/cgi/content/full/97/7/3526</a>
  • Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. <a href="#">http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491</a>